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| Registros recuperados : 9 | |
| 2. |  | SCHILD, C.; ARMENDANO, J.I.; LIBOREIRO, M.; BRESKY, F.; MORRELL, E.; ODRIOZOLA, E.; CANTÓN, G. Caudal vena cava thrombosis in a dairy cow (Bos taurus) in Argentina. [Trombose da veia cava caudal em uma vaca leiteira (Bos taurus) na Argentina.] Ciência Rural, 47(6), e20160917. Epub May 04, 2017. Article history: Received: October 05, 2016; Accepted: March 08, 2017; Revised: April 10, 2017.| Biblioteca(s): INIA Las Brujas. |
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| 4. | | GIANNITTI, F.; GARCÍA, J.P.; ROOD, J.I.; ADAMS, V.; ARMENDANO, J.I.; BEINGESSER, J.; UZAL, F.A. Cardiopulmonary Lesions in Sheep Produced by Experimental Acute Clostridium Perfringens Type D Enterotoxemia. Veterinay Pathology, volumen 58, number 1, pag. 103-113, 2021. Doi: https://doi.org/10.1177/0300985820965554 Article history: First Published October 15, 2020. Corresponding Author:Francisco A. Uzal, Email: fuzal@cahfs.ucdavis.edu. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or...| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | SILVEIRA, C.S.; ARMENDANO, J.I.; MOORE, D.P.; CANTÓN, G.J.; MACÍAS-RIOSECO, M.; RIET-CORREA, F.; GIANNITTI, F. A comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay]. Revista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004 Article history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019.
Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de...| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 6. | | MACÍAS-RIOSECO, M.; ARMENDANO, J.I.; COSTA, R.A. DA; FRAGA, M.; CAFFARENA, D.; ARÁOZ, V.; PLA, M.; GIANNITTI, F.; RIET-CORREA, F. Caída en la producción láctea, signos respiratorios agudos y muertes asociadas a estrés calórico en bovinos lecheros de Uruguay.(Drop in milk production, acute respiratory signs and deaths associated with heat stress in dairy cattle in Uruguay). Veterinaria (Montevideo), 2018, v. 55 n. 209. p.4-8. Article History: Recibido: 21/09/2017 // Aceptado: 21/02/2018.| Biblioteca(s): INIA La Estanzuela. |
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| 7. | | SCHILD, C.; BOABAID, F.M.; OLIVEIRA, L.G.S.; ARMENDANO, J.I.; SARAVIA, A.; CUSTODIO, A.; ALGORTA, J.; ALVAREZ, C.; JAURENA, M.; DIXON, R.M.; RIET-CORREA, F. Response of cows with osteomalacia grazing sub-tropical native pastures to phosphorus supplementation with loose mineral mix or feed blocks. Veterinary Journal. 2023, Volume 298-299, 106013. https://doi.org/10.1016/j.tvjl.2023.106013 Article history: Accepted 20 June 2023; Available online 22 June 2023. -- Correspondence author: Riet-Correa, F.; Programa de pós-graduação em Ciência Animal nos Trópicos, Escola de Medicina Veterinária e Zootecnia, Universidade Federal...| Biblioteca(s): INIA Las Brujas. |
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| 8. | | RODRIGUEZ, A.M.; SCHILD, C.; CANTÓN, G.J.; RIET-CORREA, F.; ARMENDANO, J.I.; CAFFARENA, D.; BRAMBILLA, E.C.; GARCÍA, J.A.; MORRELL, E.L; POPPENGA, R.; GIANNITTI, F. White muscle disease in three selenium deficient beef and dairy calves in Argentina and Uruguay. (Doença do músculo branco em três bezerros para carne e leite deficientes em selênio na Argentina e Uruguai). Ciência Rural, Santa Maria, v.48, 2018. Epub May 21, 2018. Article History: Received 10.11.17//Approved 04.02.18// Returned by the author 04.28.18.| Biblioteca(s): INIA La Estanzuela. |
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| 9. | | LOUGE URIARTE, E.; GONZÁLEZ PASAYO, R.; MASSÓ, M.; CARRERA PAÉZ, L.; DOMÍNGUEZ MONCLA, M.; DONIS, N.; MALENA, R.; MÉNDEZ, A.; MORRELL, E.; GIANNITTI, F.; ARMENDANO, J.I.; FAVERIN, C.; CENTRÓN, D.; PARREÑO, V.; ODEÓN, A.C.; QUIROGA, M.P.; MOREIRA, A.R. Molecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia. Microbial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378 Article history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022.
Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria...| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 9 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/01/2022 |
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Actualizado : |
25/01/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
LOUGE URIARTE, E.; GONZÁLEZ PASAYO, R.; MASSÓ, M.; CARRERA PAÉZ, L.; DOMÍNGUEZ MONCLA, M.; DONIS, N.; MALENA, R.; MÉNDEZ, A.; MORRELL, E.; GIANNITTI, F.; ARMENDANO, J.I.; FAVERIN, C.; CENTRÓN, D.; PARREÑO, V.; ODEÓN, A.C.; QUIROGA, M.P.; MOREIRA, A.R. |
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Afiliación : |
ENRIQUE L. LOUGE URIARTE, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; RAMÓN A. GOZÁLEZ PASAYO, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; MARIANA MASSÓ, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; LAURA CARRERA PAÉZ, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; MANUEL DOMÍNGUEZ MONCLA, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; NICOLÁS DONIS, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; ROSANA MALENA, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; ALEJANDRA MÉNDEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; ELEONORA MORRELL, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOAQUÍN I. ARMENDANO, Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco s/n, Tandil, 7000, Argentina; CLAUDIA FAVERIN, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; DANIELA CENTRÓN, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; VIVIANA PARREÑO, Incuinta, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Virología e Innovaciones Tecnológicas, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IVIT, INTA-CONICET), Castelar, Buenos Aires, 1712, Argentina; ANSELMO C. ODEÓN, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; MARÍA PAULA QUIROGA, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; ANA RITA MOREIRA, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina. |
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Título : |
Molecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Microbial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378 |
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ISSN : |
0882-4010 |
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DOI : |
10.1016/j.micpath.2021.105378 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022.
Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Ruta 226 km 73.5, Balcarce, Buenos Aires, Argentina; email:lougeuriarte.enrique@inta.gob.ar --- Quiroga, M.P.; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina; email:paula.quiroga@conicet.gov.ar -- |
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Contenido : |
ABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect 19 VGs by uniplex PCR. Highly diverse VG profiles were characterized among NMEC and SEPEC isolates, but iucD was the predominant virulence marker. Histologic lesions in all calves supported their pathogenicity. Selected isolates mainly belonged to phylogroups A and C and showed multidrug resistance. Classic (dfrA17 and arr3-dfrA27) and complex (dfrA17-aadA5::ISCR1::blaCTX-M-2) class 1 integrons were identified. Target-site mutations in GyrA (S83L and D87N) and ParC (S80I) encoding genes were associated with FQ resistance. The VGs detected more frequently in fecal samples included f17G (50%), papC (30%), iucD (20%), clpG (19%), eae (16%), and afaE-8 (13%). Fecal isolates displaying the profiles of f17 or potential SEPEC were found in 25% of calves with and without diarrhea. The frequency of E. coli VGs and profiles did not differ between both groups (p > 0.05) and were identical or similar to those found in NMEC and SEPEC. Overall, multidrug-resistant E. coli isolates with diverse VG profiles and belonging to phylogroups A and C can be implicated in natural cases of meningitis and septicemia. Their resistance phenotypes can be partially explained by class 1 integron gene cassettes and target-site mutations in gyrA and parC. These results highlight the value of antimicrobial resistance surveillance in pathogenic bacteria isolated from food-producing animals. Besides, calves frequently shed potential SEPEC in their feces as commensals (?Trojan horse?). Thus, these bacteria may be disseminated in the farm environment, causing septicemia and meningitis under predisposing factors.
© 2022 Elsevier Ltd MenosABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect... Presentar Todo |
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Palabras claves : |
CALVES; E. COLI; Integrons; Multidrug resistance; Phylogroups; Quinolone resistance-determining region (QRDR) mutations; Virulence genes. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
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100 1 $aLOUGE URIARTE, E.
245 $aMolecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022. Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Ruta 226 km 73.5, Balcarce, Buenos Aires, Argentina; email:lougeuriarte.enrique@inta.gob.ar --- Quiroga, M.P.; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina; email:paula.quiroga@conicet.gov.ar --
520 $aABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect 19 VGs by uniplex PCR. Highly diverse VG profiles were characterized among NMEC and SEPEC isolates, but iucD was the predominant virulence marker. Histologic lesions in all calves supported their pathogenicity. Selected isolates mainly belonged to phylogroups A and C and showed multidrug resistance. Classic (dfrA17 and arr3-dfrA27) and complex (dfrA17-aadA5::ISCR1::blaCTX-M-2) class 1 integrons were identified. Target-site mutations in GyrA (S83L and D87N) and ParC (S80I) encoding genes were associated with FQ resistance. The VGs detected more frequently in fecal samples included f17G (50%), papC (30%), iucD (20%), clpG (19%), eae (16%), and afaE-8 (13%). Fecal isolates displaying the profiles of f17 or potential SEPEC were found in 25% of calves with and without diarrhea. The frequency of E. coli VGs and profiles did not differ between both groups (p > 0.05) and were identical or similar to those found in NMEC and SEPEC. Overall, multidrug-resistant E. coli isolates with diverse VG profiles and belonging to phylogroups A and C can be implicated in natural cases of meningitis and septicemia. Their resistance phenotypes can be partially explained by class 1 integron gene cassettes and target-site mutations in gyrA and parC. These results highlight the value of antimicrobial resistance surveillance in pathogenic bacteria isolated from food-producing animals. Besides, calves frequently shed potential SEPEC in their feces as commensals (?Trojan horse?). Thus, these bacteria may be disseminated in the farm environment, causing septicemia and meningitis under predisposing factors. © 2022 Elsevier Ltd
653 $aCALVES
653 $aE. COLI
653 $aIntegrons
653 $aMultidrug resistance
653 $aPhylogroups
653 $aQuinolone resistance-determining region (QRDR) mutations
653 $aVirulence genes
700 1 $aGONZÁLEZ PASAYO, R.
700 1 $aMASSÓ, M.
700 1 $aCARRERA PAÉZ, L.
700 1 $aDOMÍNGUEZ MONCLA, M.
700 1 $aDONIS, N.
700 1 $aMALENA, R.
700 1 $aMÉNDEZ, A.
700 1 $aMORRELL, E.
700 1 $aGIANNITTI, F.
700 1 $aARMENDANO, J.I.
700 1 $aFAVERIN, C.
700 1 $aCENTRÓN, D.
700 1 $aPARREÑO, V.
700 1 $aODEÓN, A.C.
700 1 $aQUIROGA, M.P.
700 1 $aMOREIRA, A.R.
773 $tMicrobial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378
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